Figure S2.
Nuclear measurements in kuk disrupted embryos. (A) Fold change of expression of Rh3 and kuk using two different primers obtained by normalizing with sqh (positive) control. (B) Comparison of 3D long-axis length of nuclei in control (luciferase shRNA) and kuk embryos. (C) Comparing nucleus width at nuclear midplane for control and kuk embryos; n = 9,611 nuclei and n = 315 nuclei for kuk from k = 3 embryos for each background. (D) Comparison of nuclear volume in control and kuk embryos. For B and D, n = 961 nuclei for control and n = 580 nuclei for kuk from k = 3 embryos for each background. (E) Cell area measured at apical region (z-depth = 7 μm from the apical surface) in kuk embryos demonstrating increased variation in cell areas at the later time point of GBE indicated by wider whiskers at 20 min; n = 285 cells from k = 3 embryos. (E′) Comparison of SD of cell area (z-depth = 7 μm from apical surface) in control and kuk embryos from the onset (0 min) to 20 min after the onset of GBE; n = 961 cells for control and n = 580 for kuk from k = 3 embryos. (F) Ratio of nucleus area to cell area at the nuclear midplane in kuk embryos compared at −5 min and +20 min of GBE; n = 285 cells and nuclei from k = 3 embryos. (G) Relative myosin intensity at horizontal (75–90° interface angle) and vertical (0–15° interface angle) interfaces showing myosin polarity in control and kuk embryos; n = 2,723 interfaces for control, n = 2,827 interfaces for kuk from k = 3 embryos for each background. Insets show rose plots showing directionality of contracting interface; n = 1,601 interfaces for control and n = 1,387 interfaces for kuk from k = 3 for each background. (H) Probability distribution of medial myosin intensities (per square microns) in wildtype and kuk embryos. The box chart in the inset shows no significant changes in myosin intensities due to kuk disruption; n = 1,011 cells for wildtype and n = 882 cells for kuk from k = 3 embryos for each background. The measured n values are regardless of time points where not indicated. For F, statistical significance was calculated using Student’s t test. For A–C, H, and I, statistical significance was calculated using the Mann–Whitney U-test. ns = not significant, ***P < 0.001, ****P < 0.0001.

Nuclear measurements in kuk disrupted embryos. (A) Fold change of expression of Rh3 and kuk using two different primers obtained by normalizing with sqh (positive) control. (B) Comparison of 3D long-axis length of nuclei in control (luciferase shRNA) and kuk embryos. (C) Comparing nucleus width at nuclear midplane for control and kuk embryos; n = 9,611 nuclei and n = 315 nuclei for kuk from k = 3 embryos for each background. (D) Comparison of nuclear volume in control and kuk embryos. For B and D, n = 961 nuclei for control and n = 580 nuclei for kuk from k = 3 embryos for each background. (E) Cell area measured at apical region (z-depth = 7 μm from the apical surface) in kuk embryos demonstrating increased variation in cell areas at the later time point of GBE indicated by wider whiskers at 20 min; n = 285 cells from k = 3 embryos. (E′) Comparison of SD of cell area (z-depth = 7 μm from apical surface) in control and kuk embryos from the onset (0 min) to 20 min after the onset of GBE; n = 961 cells for control and n = 580 for kuk from k = 3 embryos. (F) Ratio of nucleus area to cell area at the nuclear midplane in kuk embryos compared at −5 min and +20 min of GBE; n = 285 cells and nuclei from k = 3 embryos. (G) Relative myosin intensity at horizontal (75–90° interface angle) and vertical (0–15° interface angle) interfaces showing myosin polarity in control and kuk embryos; n = 2,723 interfaces for control, n = 2,827 interfaces for kuk from k = 3 embryos for each background. Insets show rose plots showing directionality of contracting interface; n = 1,601 interfaces for control and n = 1,387 interfaces for kuk from k = 3 for each background. (H) Probability distribution of medial myosin intensities (per square microns) in wildtype and kuk embryos. The box chart in the inset shows no significant changes in myosin intensities due to kuk disruption; n = 1,011 cells for wildtype and n = 882 cells for kuk from k = 3 embryos for each background. The measured n values are regardless of time points where not indicated. For F, statistical significance was calculated using Student’s t test. For A–C, H, and I, statistical significance was calculated using the Mann–Whitney U-test. ns = not significant, ***P < 0.001, ****P < 0.0001.

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