Aβ deposits in the retina, optic nerve vasculature, and periorbital lymphatics of AD patients. (A and B) The schematic diagram of the retina and optic nerve showing the distribution of blood vessels, lymphatics, and myelinated axons. (C) The retina and optic nerve sections were stained with 6E10 (red) for Aβ, and with Laminin (green) for retinal vessels (a), the central retinal vessel of the optic nerve (b), and periorbital blood vessels (c), with IgG used as a negative control (d). White arrowheads indicated the deposition of Aβ in the microvasculature of the retina, the intermediate layer of the vascular wall, and outside the vascular wall. (D) Representative immunofluorescence images of AD patient eye tissue sections stained with 6E10 (red), LYVE1 (purple), and Laminin (green) showing Aβ deposition in the posterior eye RPE-choroid-sclera complex. (E and F) The 3D reconstructed images and fluorescence intensity line graphs demonstrate the relationship between the deposition location of Aβ and the positions of blood vessels and lymphatic vessels. (G–I) Representative immunofluorescence images of AD patient eye tissue sections stained with 6E10 (red), LYVE1 (purple), and Laminin (green) showing Aβ deposition in the (G) periorbital lymphatics, and (H) optic nerve meningeal lymphatics, as well as (I) Schlemm’s canal. Prox1 (red) and Lyve1 (purple) were used to label lymphatic vessels. White arrowheads indicated Aβ deposition within the lumen of the lymphatic vessels. (J) Representative immunofluorescence images of AD patient eye tissue sections stained with 6E10 (red), MBP (purple), and TUJ1 (green) demonstrating Aβ deposition in the spaces between myelinated axons and fascicles.