SMARCA5 is required to establish an appropriate antigen-specific B cell immune response. (A) Scheme illustrating the experimental design. (B) Representative TPLSM images of intact popliteal LNs removed at the indicated time points after NP-KLH immunization (n = 3–4; three independent experiments). Scale bar: 200 µm. (C–E) Representative flow cytometry plots and frequencies of total transferred (C), activated (D; left and middle), GC (D; right), or ASCs (E), antigen-specific B1-8hi B cells in popliteal LNs at the indicated time points after NP-KLH immunization (n = 7; two independent experiments, two-tailed Student’s t test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). Each dot in the graph represents a single mouse.
Figure 4.

SMARCA5 is required to establish an appropriate antigen-specific B cell immune response. (A) Scheme illustrating the experimental design. (B) Representative TPLSM images of intact popliteal LNs removed at the indicated time points after NP-KLH immunization (n = 3–4; three independent experiments). Scale bar: 200 µm. (C–E) Representative flow cytometry plots and frequencies of total transferred (C), activated (D; left and middle), GC (D; right), or ASCs (E), antigen-specific B1-8hi B cells in popliteal LNs at the indicated time points after NP-KLH immunization (n = 7; two independent experiments, two-tailed Student’s t test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). Each dot in the graph represents a single mouse.

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