SMARCA5 is required for B cell expansion, class-switch recombination, and ASC formation. (A) Representative flow cytometry histograms and quantification of CTV indicating the in vitro proliferation and absolute number of splenic B cells treated with LPS for 4 days (n = 5–8; two independent experiments, two-tailed Student’s t test; **P ≤ 0.01). (B) Representative flow cytometry histograms and frequencies of untreated B cells, and B cells stimulated with LPS for 16 h (top) or 4 days (bottom) (n = 5–6; two independent experiments, one-way ANOVA; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns, not significant). (C and D) Representative flow cytometry plots and frequencies of IgG1⁺ B cells (C) or total B cells and ASCs (D) derived from naive spleens that were either left unmanipulated or stimulated in vitro with LPS+IL-4 for 4 days (n = 4–6; two independent experiments, one-way ANOVA; ***P ≤ 0.001, ns, not significant). Each dot in the graphs represents a single mouse.