GC and ASC formation are dependent on SMARCA5. (A) Serum IgM, IgA, and IgG1 titers as determined by ELISA in control or Smarca5-deficient mice (n = 7–10 unmanipulated mice; two independent experiments, two-tailed Student’s t test; **P ≤ 0.01, ***P ≤ 0.001). (B) Representative flow cytometry plots and frequencies of B cell subsets in the BM of unmanipulated control versus Smarca5-deficient mice (n = 5; two independent experiments, two-tailed Student’s t test; ns, not significant). (C) Representative flow cytometry plots and frequencies of total ASCs in the BM of unmanipulated mice (n = 5; two independent experiments, two-tailed Student’s t test; *P ≤ 0.05). (D) Representative flow cytometry plots and frequencies of total B cells, ASCs, and GC B cells in popliteal LNs 7 days after NP-KLH immunization (n = 10–11; four independent experiments, two-tailed Student’s t test; ***P ≤ 0.001). (E) Representative flow cytometry plot and frequencies of GC B cells in popliteal LNs 7 days after NP-KLH immunization of chimeric mice consisting of 50% tdTomato CD45.2 WT and 50% CD45.2 Smarca5-deficient BM cells (n = 5; two independent experiments, two-tailed Student’s t test; ***P ≤ 0.001). (F) Representative flow cytometry plots and frequencies of ASCs and GC B cells in popliteal LNs 7 days after NP-KLH immunization of control and AID.Cre.Smarca4fl/fl mice (n = 7–12; three independent experiments, two-tailed Student’s t test; *P ≤ 0.05, ***P ≤ 0.001). Each dot in the graphs represents a single mouse.
Figure 2.

GC and ASC formation are dependent on SMARCA5. (A) Serum IgM, IgA, and IgG1 titers as determined by ELISA in control or Smarca5-deficient mice (n = 7–10 unmanipulated mice; two independent experiments, two-tailed Student’s t test; **P ≤ 0.01, ***P ≤ 0.001). (B) Representative flow cytometry plots and frequencies of B cell subsets in the BM of unmanipulated control versus Smarca5-deficient mice (n = 5; two independent experiments, two-tailed Student’s t test; ns, not significant). (C) Representative flow cytometry plots and frequencies of total ASCs in the BM of unmanipulated mice (n = 5; two independent experiments, two-tailed Student’s t test; *P ≤ 0.05). (D) Representative flow cytometry plots and frequencies of total B cells, ASCs, and GC B cells in popliteal LNs 7 days after NP-KLH immunization (n = 10–11; four independent experiments, two-tailed Student’s t test; ***P ≤ 0.001). (E) Representative flow cytometry plot and frequencies of GC B cells in popliteal LNs 7 days after NP-KLH immunization of chimeric mice consisting of 50% tdTomato CD45.2 WT and 50% CD45.2 Smarca5-deficient BM cells (n = 5; two independent experiments, two-tailed Student’s t test; ***P ≤ 0.001). (F) Representative flow cytometry plots and frequencies of ASCs and GC B cells in popliteal LNs 7 days after NP-KLH immunization of control and AID.Cre.Smarca4fl/fl mice (n = 7–12; three independent experiments, two-tailed Student’s t test; *P ≤ 0.05, ***P ≤ 0.001). Each dot in the graphs represents a single mouse.

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