SMARCA5 is required for proper cell division and not for cell viability. (A) SMARCA5 protein expression was determined by western blot analysis of B cells that were either left unmanipulated or stimulated with LPS. Blots show two independent biological repeats. (B) Flow cytometry histograms of all repetitions related to Fig. 3 A, and quantification of CTV in each cell division of the in vitro proliferation of B cells treated with LPS (n = 8; two independent experiments). (C) Analysis of the different cell-cycle stages in LPS stimulated B cells by EdU incorporation and 7AAD DNA staining (n = 5–6; two independent experiments, two-tailed Student’s t test; ns, not significant). (D) Flow cytometry histograms and the fraction of live or dead cells measured by the viability dye 7AAD, of LPS-stimulated splenic B cells (n = 5–6; two independent experiments, one-way ANOVA; ns, not significant). Each dot in the graph represents a single mouse. Source data are available for this figure: SourceData FS2.