GC B cells are enriched in SMARCA5 mRNA transcripts during translation. (A) Scheme illustrating the experimental design. (B and C) Volcano plots showing differential gene expression following ribosomal IP (Sort-IP) in DZ (B) and LZ (C) B cells (n = 4; two independent experiments, P value < 0.05, and log₂ FC >0.58 or less than −0.58; raw P values were adjusted for multiple testing using the procedure of Benjamini and Hochberg). (D and E) Graphs showing normalized reads of selected enriched genes in the Sort-IP group derived from DZ (D) or LZ (E) B cells (n = 4; two independent experiments, two-tailed Student’s t test). Each dot in the graphs represents a single mouse; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.