Changes in gene expression and H3K4 trimethylation levels in SETD1B-deficient cells. (A) Direct TP53 targets genes derived from downregulated signatures as shown in Fig. 4 B. (B) Heatmap displaying the differential gene expression analysis of the top significantly differentially expressed genes (Log2FC > 1, FDR < 0.05) between OCI-LY8 cells carrying sgLacZ (n = 3) or sgSETD1B (sg1 clone 1) (n = 3). (C) Computed overlay of the significantly differentially expressed genes as compared with the hallmark pathways from MsigDB. (D) Enrichment plot for P53 pathway and apoptosis hallmark gene sets of GSEA comparing OCI-LY8; sgSETD1B and OCI-LY8; sgLacZ. NES, normalized enrichment score. (E) Western blots of OCI-LY19; sgLacZ and OCI-LY19; sgSETD1B (sg1 and sg2) cell lines blotted with H3K4 mono-, di- and tri-methylated and total H3 antibodies. Data representative of two independent experiments are shown. MW, molecular weight in kD. (F) Normalized IGV read-density tracks of H3K4me3 ChIP-seq peaks at the loci of representative genes (INFGR1 and CASP1) from the ChIP-seq experiments. Signals are plotted on a normalized read per million (RPM) bases. (G) Representative western blot analysis of BIK protein expression in OCI-LY19; sgLacZ and OCI-LY19; sgSETD1B clones. β-Actin was used as a loading control. Data representative of two independent experiments are shown. MW, molecular weight in kD. Source data are available for this figure: SourceData FS4.