Rac2 A59S– and E62K–triggered IL-1β secretion is GSDMD dependent. (A) U937 NINJ1 KO cells expressing empty vector (Control), HA-RAC2 A59S, or HA-RAC2 E62K were treated with LPS for 8 h to induce the expression of pro-IL-1β. Supernatants were analyzed using ELISA for IL-1β. Data are mean ± SEM. Statistical analyses were performed using ordinary one-way ANOVA. ***P < 0.001; ****P < 0.0001. Data are representative for n = 3. (B) LDH release was measured in the culture medium of U937 GSDMD KO cells expressing empty vector (Control) or HA-tagged RAC2 A59S or RAC2 E62K. The graph represents the percentage of cytotoxicity. Data are representative for n = 3.