Figure 3.
NLRP3 inflammasome activation induced by RAC2 active mutants is inhibited by inhibitors of the PAK–NLRP3 inflammasome signaling pathway. (A–F) HEK293T cells were transfected for 16 h with plasmids encoding the NLRP3 inflammasome components (Inflam. mix) together with the HA-RAC2 Q61E (A), HA-RAC2 Q61R (B), HA-RAC2 E62K (C), HA-RAC2 D63V (D), HA-RAC2 G12R (E), or HA-RAC2 G12V (F). Inhibitors of NLRP3 (MCC950), PAK (IPA3 and AZ13711265), or pan-Caspases (Emricasan) were added 6 h before transfection. Cleaved-IL-1β secretion in the culture supernatant (Sup) and protein expression in the cell lysates were assessed by immunoblotting. Data are representative for n = 3. Molecular weight (kD) is indicated in the figure. Source data are available for this figure: SourceData F3.

NLRP3 inflammasome activation induced by RAC2 active mutants is inhibited by inhibitors of the PAK–NLRP3 inflammasome signaling pathway. (A–F) HEK293T cells were transfected for 16 h with plasmids encoding the NLRP3 inflammasome components (Inflam. mix) together with the HA-RAC2 Q61E (A), HA-RAC2 Q61R (B), HA-RAC2 E62K (C), HA-RAC2 D63V (D), HA-RAC2 G12R (E), or HA-RAC2 G12V (F). Inhibitors of NLRP3 (MCC950), PAK (IPA3 and AZ13711265), or pan-Caspases (Emricasan) were added 6 h before transfection. Cleaved-IL-1β secretion in the culture supernatant (Sup) and protein expression in the cell lysates were assessed by immunoblotting. Data are representative for n = 3. Molecular weight (kD) is indicated in the figure. Source data are available for this figure: SourceData F3.

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