Excess blocking time in G ₂ increases the number of multipolar cells. (A) Representative images of monopolar, bipolar, and multipolar RPE1 CDK1^as cells. Scale bar = 5 µm. (B) Frequency of number of spindle poles (mono/bipolar and multipolar) after 8, 10, 12, and 20 h incubation with 1NM-PP1. (C) Percentage of apoptotic cells (0, 1.5, 2, and 2.5 h) after release from a 20 h incubation with 1NM-PP1. Each represents the time after release.