Figure 1. Schematic of Ca v1.2 α 1C and... | Rockefeller University Press

Figure 1.

$Schematic of Cav1.2α1Cand β2Bsubunits and proposed mechanism of Rad regulation. (A) Basal ICa,L is inhibited by Rad, an effect dependent on an interaction with β2B and association of Rad with the t-tubular membrane. The lipid interaction depends on helix 8, a polybasic region of the C-terminus. (B) Truncation of the Rad C-terminus by introducing a stop codon at Ala277 dissociates Rad from the t-tubule membrane and relieves ICa,L inhibition. (C) Application of isoprenaline results in Rad phosphorylation by PKA, dissociation of a Rad fraction from the t-tubule membrane and relief of ICa,L inhibition. The inset shows Rad in relation to other RGK subfamily, Ras family, and Ras superfamily members.$

Schematic of Ca _v1.2 α _1C and β _2B subunits and proposed mechanism of Rad regulation. (A) Basal I_Ca,L is inhibited by Rad, an effect dependent on an interaction with β_2B and association of Rad with the t-tubular membrane. The lipid interaction depends on helix 8, a polybasic region of the C-terminus. (B) Truncation of the Rad C-terminus by introducing a stop codon at Ala277 dissociates Rad from the t-tubule membrane and relieves I_Ca,L inhibition. (C) Application of isoprenaline results in Rad phosphorylation by PKA, dissociation of a Rad fraction from the t-tubule membrane and relief of I_Ca,L inhibition. The inset shows Rad in relation to other RGK subfamily, Ras family, and Ras superfamily members.