Correlation analysis between Piezo2 expression and radiosensitivity in the clinic. (A) The expression of Piezo2 in tumor samples from patients with rectum adenocarcinoma (READ) post-radiotherapy stratified by responders (n = 15) and non-responders (n = 20). Scale bar = 50 µm. The blue and red arrows show the expression of Piezo2 detected by immunohistochemistry in non-responder and responder, respectively. (B) The infiltration of CD8⁺ T cells in tumor samples from patients with READ after radiotherapy grouped by responders (n = 15) and non-responders (n = 18). Scale bar = 50 µm. The blue and red arrows show the expression of CD8 detected by immunohistochemistry in non-responder and responder, respectively. (C) The correlation between Piezo2 expression and CD8⁺ T cell infiltration in READ tumor tissue samples following radiotherapy (n = 28). (D and E) Normalized Piezo2 (D) and IL-15 (E) expression in PDAC tumor tissue samples collected after radiotherapy stratified by responders (n = 24) and non-responders (n = 7) in the public database. Data were represented as means ± SEM. Statistical analysis was performed by unpaired Student’s t test (A, B, and E). The correlation between Piezo2 and CD8 infiltration was analyzed by Linear regression of Correlation built in GraphPad Prism 8.0 (C). The comparisons of two nonparametric datasets were calculated by the Mann–Whitney U test (D). *P < 0.05.