The measurement of JAK2/STAT1 activation in B16F1 cells and suppression of phosphor-JAK2/STAT1 expression by JAK inhibitor in MC38 cells. Related to Fig. 5. (A) The expression of p-JAK2 and -STAT1 expression in WT and Piezo2−/− B16F1 cells with or without IR treatment was represented by three independent experiments. (B) The expression of p-JAK2 and -STAT1 in WT and Piezo2−/− MC38 cells with or without JAK inhibitor following IR treatment was shown from three independent experiments. Data were represented as means ± SEM. Statistical analysis was performed by one-way ANOVA with multiple comparison tests. *P < 0.05; **P < 0.01; ns, no significant difference. Source data are available for this figure: SourceData FS5.
Figure S5.

The measurement of JAK2/STAT1 activation in B16F1 cells and suppression of phosphor-JAK2/STAT1 expression by JAK inhibitor in MC38 cells. Related to Fig. 5. (A) The expression of p-JAK2 and -STAT1 expression in WT and Piezo2−/− B16F1 cells with or without IR treatment was represented by three independent experiments. (B) The expression of p-JAK2 and -STAT1 in WT and Piezo2−/− MC38 cells with or without JAK inhibitor following IR treatment was shown from three independent experiments. Data were represented as means ± SEM. Statistical analysis was performed by one-way ANOVA with multiple comparison tests. *P < 0.05; **P < 0.01; ns, no significant difference. Source data are available for this figure: SourceData FS5.

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