IRF-1 was increased in irradiated cells and the KO efficiency determination for IRF-1 in Piezo2−/−cells. Related to Fig. 4. (A) The assessment of IRF-1 expression in nuclear and cytoplasm in irradiated B16F1 cells was represented from two independent experiments. (B) The determination of IRF-1 expression by immunofluorescence was shown by two independent experiments. Scale bar = 40 µm. (C and D) The KO efficiency determination of shRNA silencing for IRF-1 in Piezo2−/− MC38 and Piezo2−/− B16F1 cells was shown by two independent experiments. Data were represented as means ± SEM. Statistical analysis was performed by one-way ANOVA with multiple comparison tests. *P < 0.05; ****P < 0.0001; ns, no significant difference. Source data are available for this figure: SourceData FS4.
Figure S4.

IRF-1 was increased in irradiated cells and the KO efficiency determination for IRF-1 in Piezo2 −/− cells. Related to Fig. 4. (A) The assessment of IRF-1 expression in nuclear and cytoplasm in irradiated B16F1 cells was represented from two independent experiments. (B) The determination of IRF-1 expression by immunofluorescence was shown by two independent experiments. Scale bar = 40 µm. (C and D) The KO efficiency determination of shRNA silencing for IRF-1 in Piezo2−/− MC38 and Piezo2−/− B16F1 cells was shown by two independent experiments. Data were represented as means ± SEM. Statistical analysis was performed by one-way ANOVA with multiple comparison tests. *P < 0.05; ****P < 0.0001; ns, no significant difference. Source data are available for this figure: SourceData FS4.

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