Piezo2 deficiency in tumor cells affected CD8 ⁺ T cell infiltration and differentiation within irradiated tumors. Related to Fig. 2. (A–D) Quantifications of the percentage of Ki-67⁺ (A), TCF-1⁺ (B), CD62L⁺ (C), and TOX⁺ (D) in exhausted CD8⁺ T cells from irradiated tumors on day 14 after IR were shown from two independent experiments (n = 3–9 mice per group). (E) Quantifications of PD-1⁺ in CD8⁺ T cells from irradiated tumors on day 14 after IR were shown from two independent experiments (n = 5–6 mice per group). (F and G) Quantifications of expression of Slamf6 on CD8⁺ T cells from irradiated tumors on day 7 (F) and 14 (G) after IR were shown from two independent experiments (n = 3–8 mice per group). (H–J) The percentage of CD8⁺ T cell (H), IFN-γ⁺TNF-α⁺CD8⁺ T (I), and IFN-γ⁺ CD8⁺ T (J) cells in DLNs from tumor-bearing mice was represented from two independent experiments (n = 4–6 mice per group). (K and L) MFI summary of Ki-67 (K) and the proportion of TCF-1⁺ (L) in CD8⁺ T cells in DLNs were shown from two independent experiments (n = 4–7 mice per group). (M) The determination of Piezo2 expression in MC38-OVA cells with or without gRNAs was represented by two independent experiments. (N) The assessment of Piezo2 expression in Piezo2^−/− B16F1 with or without mPiezo2-CMV-Sport6 plasmid was shown from two independent experiments. (O and P) Ca²⁺ concentration measurement in CD45⁻ cells (O) and CD8⁺ T cell infiltration (P) in CD45⁺ cells within irradiated WT, Piezo2^−/−, and Piezo2^−/−-RE tumors were shown from two independent experiments (n = 3–6 mice per group). (Q and R) The percentage of IFN-γ⁺CD8⁺ T cells (Q) and TOX⁺ among exhausted CD8⁺ T cells (R) from irradiated WT, Piezo2^−/−, and Piezo2^−/−-RE tumors was represented from two independent experiments (n = 3–6 mice per group). Data were represented as means ± SEM. Statistical analysis was performed by one-way ANOVA with multiple comparison tests. *P < 0.05; **P < 0.01, ns, no significant difference. Source data are available for this figure: SourceData FS2.