Cell size was changed after IR accompanied by the increase of Piezo2 expression and Ca ²⁺ uptake. Related to Fig. 1. (A) The cytoskeleton staining in MC38 cells with or without radiation. Scale bar = 15 µm. (B) The cytoskeleton staining in B16F1 cells with or without radiation. Scale bar = 30 µm. (C and D) MFI of FSC-A tdTomato⁺ tumor cells from tumors on day 3 (C) and 6 (D) after IR were represented from three independent experiments (n = 4–6 mice per group). (E and F) The assessments of Piezo2 expression in MC38 (E) and B16F1 (F) tumor cells following radiation treatment were represented from three independent experiments. (G) Determination of Ca²⁺ concentration in tdTomato⁺ cells from irradiated tumors was shown from two independent experiments (n = 8–9 mice per group). (H) Determinations of Piezo2 expression in MC38 (E) and B16F1 (F) cells with or without gRNA were represented from three independent experiments. (I) Determinations of MC38 (left) and B16F1 (right) cell viability with or without Piezo2 by CCK-8 were shown from two independent experiments. Data were represented as means ± SEM. The comparisons of two nonparametric datasets were calculated by Mann–Whitney U test (A and B). C–G were analyzed by unpaired Student’s t test. *P < 0.05; **P < 0.01; ****P < 0.0001; ns, no significant difference. Source data are available for this figure: SourceData FS1.