Large-scale EM reveals novel cilia disassembly intermediates in differentiating GCs. (A–D) Centrioles in differentiating GCs had ciliary vesicles. (E and F) Centrioles with plasma membrane invaginations attached to distal appendages. (G and H) Rare tethered centrioles (G), and cytoplasmic unciliated centrioles (H). (I) Unciliated mother centriole docked at the plasma membrane. For each centriole, raw and annotated representative EM sections are displayed to the left of the 3D reconstruction (yellow: ciliary vesicles; blue: cell boundary; purple: centriole). (J) The percentage of GCs in the IGL with centrioles in each classification are quantified. Scale bars are 200 nm.