Protein level and functional analysis of MTS–containing fusion proteins. (A) Representative max projections of widefield fluorescence microscopy images of yeast expressing Tom70–mCherry and the indicated sfGFP-tagged strains examined from the N-terminal SWAp-Tag (N-SWAT) library all treated with 200 nM Rap. MDCs are indicated by the white arrows. Scale bar = 2 µm. (B) Immunoblots of whole-cell protein extracts from yeast expressing the indicated proteins. Pgk1 is shown as a loading control. (C) Cell growth spot assays of cox7∆ yeast transformed with the indicated Cox7 constructs or empty vector expressed from a low-copy (pRS416) plasmid serially diluted and plated on either rich media agar plates containing dextrose (YPAD) or glycerol (YPAG). (D) Cell growth spot assays of leu4∆oac1∆ yeast transformed with the indicated Oac1 constructs expressed from a low-copy (pRS416) plasmid serially diluted and plated on SD agar plates lacking either uracil (SD-Ura) or uracil and leucine (SD–Ura–Leu). (E) Cell growth spot assays of wild-type or tom70∆ yeast transformed with the indicated Tom70 constructs or empty vector expressed from a low-copy (pRS416) plasmid serially diluted and plated on YPAD media agar plates and grown at either 30°C or 38°C. (F) Immunoblots of whole-cell protein extracts from yeast expressing the indicated proteins from the indicated promoters. Pgk1 is shown as a loading control. Source data are available for this figure: SourceData FS1.