Arid5a RIP-Seq analysis in RTECs (associated with Figs. 5 and 6). (a) HK-2 cells were transfected with Flag-Arid5a, treated ± IL-17 for 3 h (n = 3). Lysates were immunoprecipitated with anti-Flag Abs or IgG controls and subjected to Illumina sequencing. PCA showing sample heterogeneity among groups captured by gene expression. (b) Biotype distribution of 2788 Arid5a associated RNAs enriched in IL-17–treated group. (c) Genes in Gene Ontology Translation geneset (GO:0006412) in IL-17–treated Arid5a (anti-Flag) pulldowns compared with untreated controls. (d) HK-2 and HK-2^ΔARID5A cells were treated with IL-17 for 1 h, and cytoplasmic lysates were subjected to sucrose gradient fractionation. Fractions used for qPCR are indicated. (e) Translation efficiency for the indicated transcripts was calculated as the percentage of RNA compared to total RNA in large polysomes (fractions 11–13) or small polysomes (fractions 6–10)/non-polysome (fractions 2–5). Data are representative of two independent experiments. Supt, supernatant.