Arid5a-mediated C/EBP regulation (associated with Fig. 4 ). (a) Densitometric quantitation of blots (Fig. 4 b) was determined by ImageJ. Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test. C/EBPβ isoforms: LAP/LAP*, liver enriched activator protein; LIP, liver enriched inhibitor protein. (b) HK-2 cells were stimulated with human IL-17 for the indicated times, and expression of mRNAs implicated in AGN was measured by qPCR normalized to GAPDH. Each number represents an individual sample, pooled from up to six independent experiments (n = 2–6). Data are mean ± SEM, analyzed by one-way ANOVA with post-hoc Dunnett’s test, comparing each time point to the control (0 h) sample. (c) RNA stabilization was determined by Roadblock-PCR (Watson et al., 2020) in HK-2 and HK-2^ΔARID5A cells ± IL-17 (200 ng/ml) for the indicated times. Data analyzed by one phase decay best-fit. (d) HK-2 and HK-2^ΔARID5A cells were treated with human IL-17 at the indicated times. Left: Cytoplasmic or nuclear RNA assessed by qPCR, normalized to GAPDH (cytoplasm) or MALAT1 (nucleus). Right: Validation of cellular fractionation was confirmed by immunoblotting for YY1 (nucleus) or tubulin (cytoplasm). Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test. Each symbol represents an individual sample (n = 6), pooled from two independent experiments. *P < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. Source data are available for this figure: SourceData FS3.