Arid5a regulates C/EBP transcription factors. (a) The indicated mice were administered PBS or subjected to AGN. Expression of IL-17–induced TFs in AGN kidney was assessed on day 7 by qPCR normalized to Gapdh. Each symbol represents one mouse (n = 5–7) pooled from two independent experiments. Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. (b) The indicated proteins were assessed in whole kidney homogenates by immunoblotting. Each lane depicts lysates from an individual mouse. (c) Primary RTECs isolated from WT or Arid5a^−/− kidneys were stimulated with IL-17. The indicated genes were assessed by qPCR normalized to Gapdh. Each symbol represents one mouse (n = 8), pooled from six independent experiments and analyzed by one-way ANOVA with Sidak’s test for multiple comparisons for each time point. (d) Cell lysates from primary murine RTECs were subjected to immunoblotting. C/EBPβ isoforms: LAP/LAP*, liver enriched activator protein; LIP, liver enriched inhibitor protein. Data are representative of three independent experiments. (e) HK-2 and HK-2^ΔARID5A cells were stimulated with human IL-17 at the following time points: LCN2 and NFKBIZ at 2 h, IL6, CEBPB, and CEBPD at 8 h. Expression was assessed by qPCR, normalized to GAPDH. Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. Each symbol represents one sample, pooled from four independent experiments. (f) HK-2 and HK-2^ΔARID5A cells were treated with IL-17 for the indicated times, and lysates were analyzed by immunoblotting. Image representative of three independent experiments. *P < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001. Source data are available for this figure: SourceData F4.