Cell proliferation is not altered in the absence of Arid5a. (a) C57BL/6 WT and Arid5a^−/− mice were administered PBS (Control) or subjected to AGN. On day 7, renal cell proliferation was determined by staining for CD45⁻CD133⁺Ki67⁺ absolute cell number (left) and percentages (right) in the CD45⁻ population. Each symbol represents one mouse (n = 3–4). (b) BrdU was injected i.p. on 6 days after AGN induction. On day 7, renal cell homogenates were assessed by flow cytometry. CD133⁻CD45⁺ (top) and CD133⁺CD45⁻ (bottom). (c) Percentage of cells within the indicated cell cycle stages in CD133⁺CD45⁻ (left) and CD133⁻CD45⁺ (right) populations were quantified. Data analyzed by ordinary one-way ANOVA with post-hoc Tukey’s test. Each symbol represents one mouse (n = 2–3). (d) Representative Ki-67 staining in WT or Arid5a^−/− primary fibroblasts isolated from mouse ear and treated ± IL-17 (200 ng/ml) for 3 or 6 h. Plot is representative of three independent experiments. (e) WT and Arid5a^−/− primary ear fibroblasts were plated and recovered in a standard scratch assay was monitored at the indicated time points. Each number represents one monitored area (n = 18), pooled from six independent samples. Data analyzed by two-way ANOVA with post-hoc Tukey’s test with no significant differences observed between WT and Arid5a^−/− cells. Representative images are shown. Scale bar = 400 µm.