Arid5a is required for AGN pathology. The indicated mice were administered PBS (Control) or subjected to AGN. (a) Top: Serum BUN and creatinine were evaluated by ELISA on day 14. Each symbol represents one mouse (n = 5–8) pooled from two independent experiments. Bottom: H&E staining of kidney sections with clinical scoring. Representative images are shown. Size bar = 200 µm. Percent of abnormal glomeruli within each field is indicated. Each symbol represents one mouse (n = 3–4). Data are mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. (b) Indicated mRNAs in kidney were assessed on day 7 by qPCR normalized to Gapdh. Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. Each symbol represents one mouse (n = 5–7) pooled from two independent experiments. (c) C57BL/6 WT and Arid5a^−/− mice were administered PBS (Control) or subjected to AGN. Kidneys were analyzed on day 7. Representative flow cytometry plots and percentages are shown for macrophages (CD45⁺CD11b⁺F4/80⁺) and inflammatory monocytes (CD45⁺CD11b⁺Ly6C⁺Ly6G⁻). Each symbol represents one mouse (n = 5–7), pooled from two independent experiments. Data were analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. (d) C57BL/6 WT or Arid5a^−/− mice were lethally irradiated and reconstituted with femoral BM from reciprocal donors. After 6 wk, mice were administered PBS (Control) or subjected to AGN. On day 14, serum BUN and creatinine were measured by ELISA. Each symbol represents one mouse (n = 7–8) pooled from two independent experiments. Data analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. *P < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.