Arid5a is elevated in human and mouse renal epithelium during AGN. (a) The European Renal cDNA Biobank database was interrogated for ARID5A and IL17A mRNA in the following populations (both sexes): IgA nephropathy (n = 27); focal segmental glomerulosclerosis (FSGS; n = 18); membranous nephropathy (MN, n = 21); ANCA (n = 22); tumor nephrectomy (n = 5); and healthy control (n = 21). Each symbol indicates one subject, analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons, comparing each column to healthy controls. (b) Left: Renal biopsies from patients with ANCA-associated GN or healthy controls were stained with a custom ARID5A probe. Red dots indicate amplified ARID5A mRNA. Representative images are shown. Size bar = 100 µm. Right: Quantitation of RNAScope images. Each symbol represents one individual (control, n = 4; ANCA n = 12). Data show mean ± SEM, analyzed by Student's t test. (c) Timeline of AGN model. IF, immunofluorescence. (d) C57BL/6 WT and Act1−/− mice were administered PBS (Control) or subjected to AGN. Indicated kidney mRNAs were assessed on day 7 by qPCR normalized to Gapdh. Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. Each symbol represents one mouse (n = 4–7), pooled from two independent experiments. (e) Frozen kidney sections from WT mice subjected to AGN for 7 days were stained for DAPI and Arid5a. Representative images indicating renal cortex and medulla are shown. Size bar = 100 µm. *P < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.
Figure 1.

Arid5a is elevated in human and mouse renal epithelium during AGN. (a) The European Renal cDNA Biobank database was interrogated for ARID5A and IL17A mRNA in the following populations (both sexes): IgA nephropathy (n = 27); focal segmental glomerulosclerosis (FSGS; n = 18); membranous nephropathy (MN, n = 21); ANCA (n = 22); tumor nephrectomy (n = 5); and healthy control (n = 21). Each symbol indicates one subject, analyzed by one-way ANOVA with Dunnett’s test for multiple comparisons, comparing each column to healthy controls. (b) Left: Renal biopsies from patients with ANCA-associated GN or healthy controls were stained with a custom ARID5A probe. Red dots indicate amplified ARID5A mRNA. Representative images are shown. Size bar = 100 µm. Right: Quantitation of RNAScope images. Each symbol represents one individual (control, n = 4; ANCA n = 12). Data show mean ± SEM, analyzed by Student's t test. (c) Timeline of AGN model. IF, immunofluorescence. (d) C57BL/6 WT and Act1−/− mice were administered PBS (Control) or subjected to AGN. Indicated kidney mRNAs were assessed on day 7 by qPCR normalized to Gapdh. Mean ± SEM, analyzed by one-way ANOVA with post-hoc Tukey’s test for multiple comparisons. Each symbol represents one mouse (n = 4–7), pooled from two independent experiments. (e) Frozen kidney sections from WT mice subjected to AGN for 7 days were stained for DAPI and Arid5a. Representative images indicating renal cortex and medulla are shown. Size bar = 100 µm. *P < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001.

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