Localization of gs2::YFP and pdi::YFP transcripts in Drosophila glia. (A) Representative image of a Drosophila third instar larva NMJ (Segment A4), showing the neuron and muscle cell nuclei in cyan (anti-HRP antibody conjugated to Alexa-405 fluor and DAPI, respectively), gs2::YFP protein in green, glial membrane of perisynaptic glia labeled with Repo>mCD8-mCherry in magenta, and the YFP exon associated with gs2::YFP protein in white (ATTO633). (B–D) A single-channel view (see label) of a zoomed-in area (i) of (A) in which multiple mRNA molecules are present in the glial projection. (E) A zoomed in area (ii) of (A) showing the entire length of the perisynaptic glia at that synapse with the mRNA molecules distributed regularly throughout the glial cell projections. (F–I) Confocal image of third instar larval NMJ (Segment A4) of Pdi::YFP genotype. The same color scheme as (A) is used. Circular regions of interest in G–I represent individual clusters of Pdi::YFP mRNA localizing to the glial protrusion membrane.