Global cytokine responses in in vitro–activated T cells from patients. (A) RNA-seq analysis of in vitro–activated T cells from patients (n = 5) compared with HCs (n = 3) in unstimulated condition or upon IL-2 or IFN-α stimulation for 3 h. Significant pathways enrichment in patients (A1, A2, B1, C1, and D1) on a list of 36 pathways from MSigDB database are represented. NS, nonstimulated. (B) Significantly upregulated pathways from NCI Nature database in in vitro–activated T cells from patients (n = 5) (A1, A2, B1, C1, and D1) as compared with HCs (n = 3) after IL-2 stimulation (10⁴ U/ml for 3 h). *P < 0.05, **P < 0.01. (C) Heatmap of the normalized gene expressions found upregulated in the signaling events mediated by PTPN2 for patients (A1, A2, B1, C1, and D1) and HC cells after IL-2 stimulation. (D) Heatmap of cytokine dosage in supernatant of resting or activated T cells stimulated by IFN-α or IL-2 (10⁴ U/ml) for 24 h. Z-score of log10 cytokine levels normalized to HC means in indicated stimulation conditions. Data from n = 2 independent experiments including a total of 13 HCs and 6 patients.