PTPN2 expression following PBMC stimulation and intracellular localization. (A) PTPN2 protein in PBMCs stimulated 6 h with indicated cytokines or ligands was quantified using automated quantitative western blot. Protein quantification was calculated on anti-PTPN2 expression normalized on total protein expression. Mean and SDs are shown and representative of three different experiments. All MW are in kDa. (B) PTPN2 mRNA was measured in control PBMCs stimulated 6 h with indicated cytokines or ligands. Mean and SDs are shown and representative of three different experiments. (C) Confocal microscopy analysis of PTPN2 subcellular localization. Constructs were overexpressed in HEK293T cells and detected with anti-HA antibody together with endoplasmic reticulum and nucleus staining. Source data are available for this figure: SourceData FS1.