PTPN2 regulation and expression. (A) PTPN2 protein expression in human primary T (LT) and B lymphocytes (LB), NK cells, monocytes, Jurkat–T cell line, and EBV-derived control B cell lines (LCL). PTPN2 expression was analyzed using automated quantitative western blot. Relative expression of TC48 and TC45 isoforms in Jurkat–T cells is indicated in the lower panel (mean and SDs from five different experiments are represented). (B) PTPN2 expression in HEK293T cell transfected with different constructs. Protein expression was revealed with anti-HA (left panel) or anti-PTPN2 (right panel) antibodies and analyzed using automated quantitative western blot. Protein quantification was calculated on anti-HA expression normalized on total protein expression. Mean and SDs are shown and representative of at least three different experiments. (C) Endogenous PTPN2 protein expression in control and patient in vitro–activated T cells analyzed using automated quantitative western blot. Quantification was calculated on anti-PTPN2 expression normalized on total protein expression. Mean and SDs are shown and representative of three different experiments (ns, non-significant, *P < 0.05, **P < 0.01, one-way analysis of variance, Kruskal–Wallis test). (D) Real-time RT-QPCR assays of PTPN2 in control and patient in vitro activated T cells. Results represent the PTPN2 expression normalized to GAPDH expression, related to the expression of controls. Mean and SDs are shown and representative of at least three different experiments (ns, nonsignificant, *P < 0.05, **P < 0.01, ****P < 0.0001, one-way analysis of variance, Kruskal–Wallis test). All MW are in kDa. Source data are available for this figure: SourceData F2.