Montages of phase shift between pairs of polarity proteins. (A) Montage of phase shift between Scd1-tdTomato and Cdc42 activity (CRIB-3xGFP) and between Scd2-mCherry and CRIB-3xGFP). Arrows denote peaks in intensity. (B) Montage of phase shift between Pak1-mEGFP and the positive feedback proteins: Scd1-tdTomato, active Cdc42 (CRIB-mCherry), and Scd2-mCherry. Arrows indicate peaks in intensity Scale bar, 5 µm. (C) FRAP analysis of Scd1-mNG, Scd2-GFP, and Pak1-mEGFP recovery after photobleaching half the cell end. Magenta boxes indicate the half of the cell end that has been photobleached. Magenta arrows show when half of the final fluorescence has recovered. Blue arrows indicate when the fluorescence recovery plateaus. Scale bar 10 µm. Phase shift montages clarified and denoised, made in Nikon NIS elements.