![AC expression and SBP-1 regulation of pod-2, fasn-1, elo-1, lpin-1, and sms-1. (A) A schematic diagram showing the role of the transcription factor SBP-1 in regulating phospholipid and sphingomyelin synthesis in the AC (refer to Fig. S1 A for complete pathway and Table S2 for RNAi screen results). (B) Sum intensity z-projection images of POD-2 (GFP::POD-2) and FASN-1 (FASN-1::mNG) in control and sbp-1 RNAi animals at the P6.p 2-cell stage. POD-2 and FASN-1 are in the AC cytoplasm (arrows, ACs). (C) Sum intensity z-projections of ELO-1 (ELO-1::mNG, ACs outlined with white dashes) and LPIN-1 (mNG::LPIN-1, arrows, ACs) in control and sbp-1 RNAi animals at the P6.p 2-cell stage. ELO-1 is localized to the ER (see Fig. S3 B) and LPIN-1 in the cytosol. (D) Sum intensity z-projection of SMS-1 (SMS-1::mNG) in control and sbp-1 RNAi animals at the P6.p 2-cell stage (ACs outlined with white dashes). SMS-1 localizes to the Golgi (see Fig. S3 C). (B–D) Right: Boxplots show the mean fluorescence intensity of each protein in the AC of control and sbp-1 RNAi treated animals ([POD-2] n = 10 control and 10 sbp-1 RNAi animals; [FASN-1] n = 22 control and 23 sbp-1 RNAi animals; [ELO-1] n = 15 control and 16 sbp-1 RNAi animals; [LPIN-1] n = 11 control and 10 sbp-1 RNAi animals; [SMS-1] n = 24 control and 13 sbp-1 RNAi animals, ** P ≤ 0.01, **** P ≤ 0.0001, ns [not statistically significant], P > 0.05, unpaired two-tailed Student’s t test and Mann–Whitney U test). All data are from two or more replicates. Scale bar, 5 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/223/10/10.1083_jcb.202402035/1/jcb_202402035_fig3.png?Expires=1768355730&Signature=wvGHX0yYmwNlSgq~oyaukQ2Sbc~yxPRvjXwiy-~3A-HqAV~kf0cZnyt1-Wq-Ac3inbPRPCb5eplb5u0JbbvtkRIgMcsZLQPvTlkmqDBvrg6REIHNtXphihs3sWSwpBYK-aLblS0z5sc0UL~Asj~izhXnUi0ixPRZKaNacq0XbN2S27EV9tcxnN5nW0ytO4-rcZC5q1t1pfXdv1hIEbzfq1aFjUhYjqqyO1cu2hCHVFEUUe~h7iW6B90cBUX4esjqIdtqy4t9no2MfzisTniI6iNA4AKZX7SqDANmY~QaPEK7y~GuFu4EUoWHMztbXQyLxUn30S~CERgBmwqz6iFnjQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
AC expression and SBP-1 regulation of pod-2, fasn-1, elo-1, lpin-1, and sms-1. (A) A schematic diagram showing the role of the transcription factor SBP-1 in regulating phospholipid and sphingomyelin synthesis in the AC (refer to Fig. S1 A for complete pathway and Table S2 for RNAi screen results). (B) Sum intensity z-projection images of POD-2 (GFP::POD-2) and FASN-1 (FASN-1::mNG) in control and sbp-1 RNAi animals at the P6.p 2-cell stage. POD-2 and FASN-1 are in the AC cytoplasm (arrows, ACs). (C) Sum intensity z-projections of ELO-1 (ELO-1::mNG, ACs outlined with white dashes) and LPIN-1 (mNG::LPIN-1, arrows, ACs) in control and sbp-1 RNAi animals at the P6.p 2-cell stage. ELO-1 is localized to the ER (see Fig. S3 B) and LPIN-1 in the cytosol. (D) Sum intensity z-projection of SMS-1 (SMS-1::mNG) in control and sbp-1 RNAi animals at the P6.p 2-cell stage (ACs outlined with white dashes). SMS-1 localizes to the Golgi (see Fig. S3 C). (B–D) Right: Boxplots show the mean fluorescence intensity of each protein in the AC of control and sbp-1 RNAi treated animals ([POD-2] n = 10 control and 10 sbp-1 RNAi animals; [FASN-1] n = 22 control and 23 sbp-1 RNAi animals; [ELO-1] n = 15 control and 16 sbp-1 RNAi animals; [LPIN-1] n = 11 control and 10 sbp-1 RNAi animals; [SMS-1] n = 24 control and 13 sbp-1 RNAi animals, ** P ≤ 0.01, **** P ≤ 0.0001, ns [not statistically significant], P > 0.05, unpaired two-tailed Student’s t test and Mann–Whitney U test). All data are from two or more replicates. Scale bar, 5 µm.