Mode shift as evidenced by tail current delay was also observed for unlabeled spHCNR332C.(A) Normalized tail currents from a representative unlabeled cell expressing spHCNR332C shown for three superfusion conditions as indicated using the voltage step protocol shown in the inset with 80-ms increments in prepulse to −160 mV. (B) Tail current delay, quantified as time to 50% of initial amplitude relative to 8 ms, plotted as a function of prepulse width for each superfusion condition.
Figure S2.

Mode shift as evidenced by tail current delay was also observed for unlabeled spHCN R332C . (A) Normalized tail currents from a representative unlabeled cell expressing spHCNR332C shown for three superfusion conditions as indicated using the voltage step protocol shown in the inset with 80-ms increments in prepulse to −160 mV. (B) Tail current delay, quantified as time to 50% of initial amplitude relative to 8 ms, plotted as a function of prepulse width for each superfusion condition.

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