Study overview. NP swabs from subjects aged 0–22 undergoing SARS-CoV-2 testing in the YNHH pediatric ED or as part of preoperative screening were collected from June 3 to July 2, 2021, and January 11 to 23, 2022 corresponding to SARS-CoV-2 low and high circulation respectively. Samples were processed and analyzed as indicated for this study.