Localization of Rab8 and Sec15 to the nascent cap. (A and B) Telophase 11 dual live imaging of PIP2 probe with endogenously-expressed Rab8-YFP (A) or with overexpressed Sec15-GFP (B). Single sections at PM folds (0 µm) and tubules (−1.5 µm). Arrows, corner brackets, and magnified insets show nascent caps. Both patterns seen in 9/9 embryos. (C) Corner brackets show Rab8 puncta at telophase 11 nascent caps with control and Cnn RNAi. Rab8 detected as overexpressed Rab8-GFP. Quantifications of Rab8 puncta numbers per nascent cap are shown. Embryo values plotted. (D) Corner brackets show Rab8 puncta at telophase 11 nascent caps with control and Arp3 RNAi. Rab8 detected as over-expressed Rab8-GFP. Quantifications of Rab8 puncta numbers per nascent cap are shown. Embryo values plotted. (E) Imaging of PIP2 probe with endogenously-expressed Rab8-YFP at telophase 11 nascent caps with control CK689 and CK666 injection. Corner brackets show nascent caps. Quantifications of Rab8 puncta numbers per nascent cap are shown. Embryo values plotted.