Effect of myosin on telophase compartment surface topography. (A–G) Telophase 11. (A) Single sections show PIP₂ probe at pseudocleavage furrows in control and Zip RNAi embryos. Time points before and after ablation are shown. Red dot indicates the ablation site. (B) Compartment areas after ablation compared to pre-ablation areas as ratios in control and Zip RNAi embryos. Embryo values plotted. (C) Maximum intensity projections with color-coded Z-positions (scale) show PM organization detected by PIP₂ probe expressed with control and Zip RNAi. The bracket shows ectopic folding between nascent caps in Zip RNAi. (D) Single sections of PIP₂ probe in control and Zip RNAi embryos. Red brackets show ectopic folds between nascent caps. Yellow arrows show abnormally deep PM tubules of nascent caps. Minimal tubules were detected below the ectopic folds. Seen in 10/10 Zip RNAi embryos. (E) Quantifications of nascent cap tubule numbers and depths in control and Zip RNAi embryos. Ectopic folds of Zip RNAi embryos were also quantified for tubules. Embryo values plotted. (F) Single sections at level of PM folds show Spider-GFP, PIP₂ probe, Rac-GTP probe, and F-actin probe with Zip RNAi. Corner brackets indicate nascent caps. Red brackets show ectopic folds. (G) Quantifications of signal ratios between nascent cap folds and ectopic folds in Zip RNAi embryos. Embryo values plotted.