Mitotic compartment responses to apical surface laser cuts. (A–C) Co-expressed Jupiter-GFP (not shown) confirmed cell cycle stages. (A) Single sections of PIP₂ probe before (magenta) and after laser cut (green). A cut of the center of the metaphase 11 compartment (yellow asterisk) produced minimal PM displacements at the apical surface or the pseudocleavage furrows below. Areas encompassed by pseudocleavage furrows of the cut compartment and of adjacent compartments quantified before and 9 s after the cut (A′). (B) Single sections of PIP₂ probe before (magenta) and after laser cut (green). A cut of the center of the telophase 11 compartment (yellow asterisk) produced PM displacements away from the cut both at the apical surface and at the pseudocleavage furrows below (green arrows). Note that adjacent compartments translocate without apparent area change. Areas encompassed by pseudocleavage furrows of the cut compartment and of adjacent compartments quantified before and 9 s after the cut (B′). (C) Single sections of PIP₂ probe before (magenta) and after laser cut (green). A cut of a nascent cap of the telophase 11 compartment (yellow asterisk) produced PM displacements away from the cut both at the apical surface and at the pseudocleavage furrows below (green arrows). Note that adjacent compartments translocate without apparent area change. Areas encompassed by pseudocleavage furrows of the cut compartment and of adjacent compartments quantified before and 9 s after the cut (C′).