Perturbation of Cnn or dynein disrupts nascent cap PM tubules. (A) Telophase 11 live imaging of Tubulin-GFP shows reduced astral centrosomal MTs with Cnn RNAi versus control RNAi (corner brackets). Seen in 5/5 embryos each. (B) Telophase 11 live imaging of Zip-GFP shows an expanded actomyosin border (green brackets) around a smaller mitotic compartment (purple outline) of Cnn RNAi embryo versus control RNAi. Seen in 9/9 Cnn RNAi embryos and 8/8 controls. (C) Sections of PIP₂ imaging at telophase 11 with control and Cnn RNAi, starting at embryo surface (0 µm). Corner brackets show nascent cap PM folds (−1.5 µm) and tubules (−3.0 µm). The purple outline shows overall telophase compartment in Cnn RNAi. The red bracket shows ectopic folds between nascent caps of Cnn RNAi compartment. Green bracket shows abnormal PM structure outside Cnn RNAi compartment. (D) Quantifications of tubule numbers and depths in embryos corresponding to C. Embryo values plotted. (E) Sections of telophase 11 PIP₂ imaging in carrier control (DMSO) and Ciliobrevin D injected embryos, starting at embryo surface (0 µm). Coexpressed Jupiter-GFP (not shown) confirmed cell cycle stage. Corner brackets show nascent cap PM folds (−1.5 µm) and tubules (−3.0 µm). (F) Quantifications of tubule numbers and depths in embryos corresponding to E. Embryo values plotted.