Dynamic proteins near Foxp3 upon IL-2 and TCR signaling. (A) Schematic of dynamic proteins near Foxp3 uncovered by Foxp3 PSI coupled with TMT-based MS. (B) Proteins enriched by SA beads in iTreg cells after IL-2 or TCR stimulation and Foxp3 PSI were resolved by SDS-PAGE followed by silver staining. Arrowhead indicates released SA. (C) Differentially represented proteins revealed by Foxp3 PSI MS after 30 min of IL-2 stimulation. Data were derived from three biological replicates. (D) Phospho-Stat5 (pStat5) staining after 30 min of IL-2 stimulation of CD4 T cells isolated from Foxp3^gfp mice. Data represent more than three experiments. Numbers show MFI of pStat5 signal. (E) Proteins enriched in Foxp3 PSI after IL-2 stimulation (Foxp3 PSI P < 0.01). Changes in total protein level are included as a comparison. (F) Differentially represented proteins revealed by Foxp3 PSI MS after 3 h of TCR stimulation. Data were derived from three biological replicates. (G) Changes in protein levels in Foxp3 PSI and whole-cell lysate upon TCR stimulation. (H) A summary of dynamic proteins revealed by Foxp3 PSI MS upon TCR stimulation without significant changes of their total levels (P > 0.05). Source data are available for this figure: SourceData F8.