Figure S2. Tracking vaccine-specific B... | Rockefeller University Press

Figure S2.

$Tracking vaccine-specific B cells in persistent GCs. Related to Fig. 2. (A) Unsupervised clustering visualized via UMAP based on scRNA-seq gene expression of all cells pooled from all blood, lymph node (LN), and bone marrow (BM) samples and time points from participants 04, 05, and 11. (B) Dot plot of the average log-normalized expression of marker genes and the fraction of cells expressing the genes in each cluster from A. (C) Annotated UMAP clusters of scRNA-seq samples pooled from blood, LN, and BM samples from all time points from participants 04, 05, and 11. (D) Dot plot for annotated clusters in C. (E) Unsupervised clustering visualized via UMAP based on scRNA-seq gene expression of cells in the B cell cluster from A, pooled from all blood, LN, and BM samples and time points from participants 04, 05, and 11. (F) Dot plot of the average log-normalized expression of marker genes and the fraction of cells expressing the genes in each cluster from E. (G) Annotated UMAP clusters of cells from the B cell cluster in E, pooled from all blood, LN, and BM samples from all time points from participants 04, 05, and 11. (H) Dot plot for annotated clusters in G. (I) Optical density (OD) at 490 nm as determined by ELISA of 2018 QIV–binding clonally unique mAbs generated from GC B cells from week 13 and week 17 from participants 05 and 04, respectively. Positive binding was defined as greater than two times the OD 490 value for antibody binding to BSA. (J) Frequency of 2018 QIV–specific GC B cell clones at the indicated time points in participant 04. Each slice represents one clonal family. The frequency of a clonal family is defined as the percentage of cells in each clonal family among the total GC B cells at each time point (n = 166 at week 1, n = 338 at week 2, n = 930 at week 17). Colored slices indicate clones identified at multiple time points.$

Tracking vaccine-specific B cells in persistent GCs. Related to Fig. 2. (A) Unsupervised clustering visualized via UMAP based on scRNA-seq gene expression of all cells pooled from all blood, lymph node (LN), and bone marrow (BM) samples and time points from participants 04, 05, and 11. (B) Dot plot of the average log-normalized expression of marker genes and the fraction of cells expressing the genes in each cluster from A. (C) Annotated UMAP clusters of scRNA-seq samples pooled from blood, LN, and BM samples from all time points from participants 04, 05, and 11. (D) Dot plot for annotated clusters in C. (E) Unsupervised clustering visualized via UMAP based on scRNA-seq gene expression of cells in the B cell cluster from A, pooled from all blood, LN, and BM samples and time points from participants 04, 05, and 11. (F) Dot plot of the average log-normalized expression of marker genes and the fraction of cells expressing the genes in each cluster from E. (G) Annotated UMAP clusters of cells from the B cell cluster in E, pooled from all blood, LN, and BM samples from all time points from participants 04, 05, and 11. (H) Dot plot for annotated clusters in G. (I) Optical density (OD) at 490 nm as determined by ELISA of 2018 QIV–binding clonally unique mAbs generated from GC B cells from week 13 and week 17 from participants 05 and 04, respectively. Positive binding was defined as greater than two times the OD 490 value for antibody binding to BSA. (J) Frequency of 2018 QIV–specific GC B cell clones at the indicated time points in participant 04. Each slice represents one clonal family. The frequency of a clonal family is defined as the percentage of cells in each clonal family among the total GC B cells at each time point (n = 166 at week 1, n = 338 at week 2, n = 930 at week 17). Colored slices indicate clones identified at multiple time points.

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