Older adult STAT3 GOF (Stat3 ^p.G421R/+ ) mice develop spontaneous skin inflammation, related to Fig. 1 . (A) Absolute cell numbers of cells per ear and of skin immune cells of older adult WT and STAT3 GOF mice. (B) Absolute cell numbers of CD45.2⁺ CD3⁺ TCRγδ⁻ CD8⁻ CD4⁺ (left) and CD45.2⁺ CD3⁺ TCRγδ^mid (right) T cells expressing RORγt, IL-17A, and/or IL-22 in the skin. (C and D) Absolute cell numbers of CD45.2⁺ CD3⁺ TCRγδ⁻ CD8⁻ CD4⁺ T cells expressing IL-17A and/or IL-22 in the (C) CLN and (D) spleen. (E and F) Representative flow plots, quantification, and absolute cell number of IL-17A, and IL-22 expression in CD45.2⁺ CD3⁺ TCRγδ⁺ T cells in the (E) CLN and (F) spleen. Cytokine expression assessed after PMA/ionomycin stimulation. Bars: mean ± SEM. Statistical significance determined by unpaired two-tailed or Welch’s t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data represent three experiments with 10–16 mice.