Assessment of BC versus HD UDCs and investigation of clinically available urine-based tests. (A) UMAP of 41,183 UDCs split by those from BC patients (40,342 cells) and those from pooled HD urine (841 cells). With a resolution of 0.4, there were 20 clusters. Cells are colored by cluster. (B) Bar plots of UDC composition in HD and BC urine. (C) Average heatmap comparing HD and BC patient UDCs expression of markers from the Cx Bladder and Xpert BC monitor tests. (D–F) Violin plots split across cells expressing individual markers comprising the (D) Cx Bladder, (E) Xpert BC Monitor, and (F) Oncuria tests. (G) Dot plot comparing expression of markers in the Oncuria urine test across BC urine samples collected during BCG therapy.