Comparison of B cells and DCs across tissue. (A) UMAP of 4,944 B cells across BC patient blood, tumor, and urine. With a resolution of 0.5, there were six clusters. Cells are colored according to cluster and annotated using published markers. (B) UMAP of B cells split by tissue. (C) Bar-plot comparison of B cell cluster composition across tissue. (D) Expression level of CXCR4 and CD83 across clusters. (E and F) Volcano plot displaying DEGs between B cells in (E) urine versus tumor and in (F) blood versus tumor. (G) Pathways differentially expressed in B cells between tumor and blood. (H) Volcano plot displaying DEGs between B cells in urine versus blood. (I) Pathways differentially expressed in B cells between urine versus blood. Cutoffs of log₂FC > |1| and P value <0.05 were for volcano plots and pathway analysis. (J) UMAP of 982 DCs across tissue. Cells are colored by cluster. (K) UMAP of DCs split by tissue. (L) Bar-plot comparison of DC subset frequency across tissue. (M) Expression levels of published DC subset gene signatures of cDC1s, cDC2s, and mregDCs, and markers of pDCs: LILRA4 and TCF4, and monocyte-derived DCs (Mono_DC): FCN1 across clusters. ESR, estrogen signaling receptor; NGF, nerve growth factor.