BC urine reflects tumor macrophage phenotypes and biology better than blood. (A) UMAP of 11,661 monocytes (Mono) and macrophages (MΦ) across BC blood, tumor, and urine. There were eight clusters identified with a resolution of 0.5. Cells are colored according to cluster and annotated based on published markers. (B) UMAP of monocytes and macrophages split by tissue. (C) Bar-plot comparison of myeloid cluster composition across tissue. (D) Expression level of markers of interest in myeloid cells across tissue. (E and F) Volcano plot displaying DEGs between monocytes and macrophages in (E) urine versus tumor and (F) in blood versus tumor. (G) Pathways upregulated in myeloid cells in tumor versus blood. (H) Volcano plot depicting DEGs between monocytes and macrophages in urine versus PBMC. (I) Pathways enriched in urine versus blood myeloid cells. Cutoffs of log₂FC > |1| and P value <0.05 were used for volcano plots and pathway analysis. (J) UMAP of 1,571 neutrophils. There were five clusters identified with a resolution of 0.3. Cells are colored by cluster. (K) UMAP of neutrophils split by tissue. (L) Bar plot comparison of neutrophil cluster composition across tissue.