Monocyte (Mono), macrophage (MΦ), NK cell, B cell, and DC characterization across tissue in BC. (A) Heatmap of monocyte and macrophage clusters demonstrating the average expression per cluster of genes used for their annotation. (B and C) (B) Volcano plot and (C) GSEA of DEGs between C1QC and SPP1 TAMs. (D) Boxplot of monocyte and macrophage cluster frequencies across tissue for all patients. (E) Venn diagram of shared genes between DEG analysis of monocyte and macrophages in tumor versus PBMC and urine versus PBMC. (F) Boxplot comparing NK cluster frequency between tissue compartments across all patients. (G) Venn diagram of overlapping DEGs of NKs in tumor versus PBMC and urine versus PBMC. (H) Expression of published signatures of lymphocyte cytolytic effector, anergy, activation, hypoxia, and glucose deprivation pathways in NK cells across tissues. Wilcoxon tests were performed (P value: * <0.05, ** <0.01, *** <0.001, **** <0.0001). (I) Dot plot of B cell annotation markers across clusters. (J) Boxplot depicting B cell cluster composition between tissue compartments across all patients. (K) Venn diagram of overlapping DEGs of B cells in tumor versus PBMC and urine versus PBMC. (L) Expression levels of CLEC9A and XCR1 across DC clusters. Ranked from highest (left) to lowest (right). (M) Boxplot displaying the frequency of DC clusters between tissue compartments across patients.