CD4 ⁺ and CD8 ⁺ T cell profiles across tissue in BC. (A) Heatmap of CD4⁺ T cell clusters demonstrating average expression per cluster of genes used for their annotation. (B) Boxplot of CD4⁺ T cell cluster frequencies between tissue across patients. (C) Venn diagram of shared genes between DEG analysis of CD4⁺ T cells in tumor versus PBMC and urine versus PBMC. (D) Expression of published gene signatures of lymphocyte cytolytic effector, anergy, activation, hypoxia, and glucose deprivation pathways in CD4⁺ T cells across tissue. (E–H) Repeat of the analyses in CD8⁺ T cell. Wilcoxon tests were performed (P value: * <0.05, ** <0.01, *** <0.001, **** <0.0001).