Ki67 is required for maintaining global chromatin accessibility in B cell progenitors undergoing gene rearrangement. (A) PCA of pre-pro-B, pro-B, and small pre-B cells from WT (n = 4) or Mki67^−/− (n = 4) mice using all peaks showing PC1 versus PC2. (B) Sankey plots showing chromatin state across stage transitions from pre-pro-B to pro-B cells (left panel) and from pro-B to small pre-B cells in WT and Mki67^−/− cells (right panel). (C) PCA of pre-pro-B, pro-B, and small pre-B cells from WT (n = 4) or Mki67^−/− (n = 4) mice using all peaks showing PC2 versus PC3. (D) Sankey plot of numbers and status of all peaks in pre-pro-B, pro-B, and small pre-B cells comparing Mki67^−/− to WT genotype. (E) Percentages of open chromatin regions altered in each chromosome in Mki67^−/− pro-B and small pre-B cells in comparison to WT cells. (F) Venn diagrams of consistently less accessible regions in both Mki67^−/− pro-B and small pre-B cells in comparison with WT cells. (G) Top 10 GO terms enriched in the genes annotated within the commonly less accessible regions in pro-B and small pre-B cells in the absence of Ki67. Data are from one experiment with four mice in WT or Mki67^−/− group.