ACh-induced PSCs were mediated by amacrine cells other than SACs. (A and B) Effects of LY354740 on ACh-induced PSCs in an ON SAC (A) and an OFF SAC (B). Open bars above the trace show the timing of the LY354740 application. Thick black bars show the timing of the ACh application. Thin black bars show the analysis time windows (20 s) of PSCs. (C and D) Summary of the effects of LY354740 on the charge transfer of ON SACs (C: Ctrl versus LY, P = 0.665; Ctrl versus LY + ACh, P = 0.0400; LY versus LY + ACh, P = 0.00749, n = 8 cells from four mice) and OFF SACs (D: Ctrl versus LY, P = 0.0156; Ctrl versus LY + ACh, P = 0.000196; LY versus LY + ACh, P = 4.68 × 10⁻⁶, n = 6 cells from five mice). Ctrl, before drug application; LY, during the LY354740 application; LY + ACh, during the ACh application in the presence of LY354740. A one-way repeated measures ANOVA and Tukey’s post hoc test were used. *P < 0.05, **P < 0.01, ***P < 0.001.