ACh-induced PSCs were mediated by non-spiking amacrine cells. (A and B) Effects of TTX on ACh-induced PSCs in an ON SAC (A) and an OFF SAC (B). Open bars above the trace show the timing of the TTX application. Thick black bars show the timing of the ACh application. Thin black bars show the analysis time windows (20 s) of PSCs. (C and D) Summary of the effects of TTX on the charge transfer of ON SACs (C: Ctrl versus TTX, P = 0.986; Ctrl versus TTX + ACh, P = 0.0124; TTX versus TTX + ACh, P = 0.0096, n = 6 cells from six mice) and OFF SACs (D: Ctrl versus TTX, P = 0.0114; Ctrl versus TTX + ACh, P = 0.615; TTX versus TTX + ACh, P = 0.00253, n = 6 cells from five mice). Ctrl, before drug application; TTX, during the TTX application; TTX + ACh, during the ACh application in the presence of TTX. A one-way repeated measures ANOVA and Tukey’s post hoc test were used. *P < 0.05, **P < 0.01.