In adult mice (≥P28), not only a nicotinic agonist, but also a muscarinic agonist increased PSCs in ON SACs. (A and C) Responses to nicotine (left) and oxotremorine (right) in ON SACs (A) and OFF SACs (C). The open bar above the trace shows the timing of the nicotine application. The thick black bar shows the timing of the oxotremorine application. Thin black bars show the analysis time windows (20 s) of PSCs. (B and D) Summary of the effects of nicotine (left) and oxotremorine (Oxo, right) on the charge transfer of ON SACs (B: nicotine, P = 0.0176, n = 6 cells [all from the whole-mount retinae] of five mice; Oxo, P = 0.0101, n = 6 cells [all from the whole-mount retinae] of five mice) and OFF SACs (D: Nicotine, P = 0.000811; Oxo, P = 0.739, n = 7 cells [six from sliced, one from whole-mount retinae] of five mice). Ctrl, before drug application; nicotine, during the nicotine application; Oxo, during the oxotremorine application. A paired t-test was used. *P < 0.05, ***P < 0.001. Oxotremorine and nicotine were applied to different cells in ON SACs because the effects of oxotremorine persisted for a long time. Nicotine and oxotremorine were applied to the same cell in OFF SACs in the order of oxotremorine followed by nicotine. Recordings were performed on adult mice (≥P28).