SR95531 blocked ACh-induced PSCs in SACs. (A and B) Effects of SR95531 on ACh-induced PSCs in ON SACs (A) and OFF SACs (B). The open bar above the trace shows the timing of the SR95531 application. Thick black bars show the timing of the ACh application. The thin black bars show the analysis time windows (20 s) of PSCs. (C and D) Summary of SR95531 effects on charge transfer in ON SACs (C: Ctrl versus SR, P = 0.000324; Ctrl versus SR + ACh, P = 0.000281; SR versus SR + ACh, P = 0.996; n = 8 cells from eight mice) and OFF SACs (D: Ctrl versus SR, P = 0.00572; Ctrl versus SR + ACh, P = 0.00582; SR versus SR + ACh, P = 1.000; n = 7 cells from five mice). Ctrl, before drug application; SR, during the SR95531 application; SR + ACh, during the ACh application in the presence of SR95531. A one-way repeated measures ANOVA and Tukey’s post hoc test were used. **P < 0.01, ***P < 0.001.